The response addressed the ISRP questions. The ISRP appreciated the effort to address the review in a professional and positive manner with explanatory notes and even figures. The ISRP expects that the principal investigators will consider the ISRP's comments on residualized hatchery fish in subsequent proposals, reports, and reviews.

A thorough response and additional references were provided, for the most part. Clearly, this is important work on the issue of wild and hatchery fish interactions and supplementation. The papers in press, in review, and planned shall become important contributions to fisheries science and particularly to the question of supplementation in the Columbia River Basin. The opportunity to review the papers in press or in review was much appreciated and assisted in confirming or addressing previous ISRP concerns quite adequately.

The question of contribution of residualized hatchery fish to parentage of wild and hatchery returns remains. Htrad may have provided no evidence of a parental contribution to returns since their success in spawning (or of progeny post-spawning) may have been near zero, but Hnew males may be more successful. The implications of reproductive success of residualized Hnew males may be substantial. It seems this could be addressed with more planning and thought, perhaps by sub-sampling residuals directly or by samples from hatchery smolts released at acclimation sites throughout the Hood River. Indeed, the opportunity may be unique to this system. Does "acclimatization" provide a benefit or loss to overall reproductive success of wild fish?

Supplementation was shown here (paper in review) to have no effect on the reproductive success of wild fish. However, does it add anything? In other words, if there is no added benefit when wild fish are seeding habitat to capacity, then what is the point of supplementation?

Ecological effects remain an issue. Regardless, a continuation of this work is highly recommended since it will address important questions on the genetics of salmonids and hatcheries, particularly if more focus is placed on the residual steelhead issue, and success in sampling can continue with the removal of the Powerdale Dam, which seems possible.

Further collaboration should be encouraged - this work should form part of a basinwide study on supplementation, filling gaps not possible in other studies and replicating work elsewhere, thus agreement on standard procedures is necessary, as appears to be unfolding.