BPA Project Number: 1994-026-00
BPA Project Title: LAMPREY RESEARCH AND RESTORATION PROJECT
Contract Number:  16223
Contract Title: LAMPREY RESEARCH AND RESTORATION PROJECT
Performance/Budget Period:   January 1, 2005 - December 31, 2005
Technical Contact Name:  Jeanette Howard
Technical Contact Title:  Project Leader
Confederated Tribes of the Umatilla Indian Reservation
Department of Natural Resource
Fisheries Program
P.O. Box 638
Pendleton, OR  97801
Technical Contact Phone:  541-966-2370
Fax:    
Technical Contact email
jhowie@uclink.berkeley.edu
JeanetteHoward@ctuir.com
jhowieca2004@yahoo.com
Contracting Contact Name:  Julie Burke
Contracting Contact Title:  Administrative contact
Confederated Tribes of the Umatilla Indian Reservation
Department of Natural Resource
Fisheries Program
P.O. Box 638
Pendleton, OR  97801
Contracting Contact Phone:  (541) 966-2372  Fax: (541) 276-4348
Contract Contact email
julieburke@ctuir.com
Financial Contact Name:  Michelle Thompson  
Financial Contact Title:  
Confederated Tribes of the Umatilla Indian Reservation
Department of Natural Resource
Fisheries Program
P.O. Box 638
Pendleton, OR  97801
Financial Contact Phone:  (541) 966-2323
Financial Contact email
michellethompson@ctuir.com

FOR CONTRACT BACKGROUND SECTION:
Introduction
Since its inception in 1995, the Lamprey Research and Restoration Project has focused on two major components: 1) restoration activities involving outplanting broodstock from the mainstem Columbia to increase larval lamprey abundance in the upper Umatilla River; and 2) understanding the biology and ecology of Pacific lamprey. In the coming year we propose to expand both of these efforts to bring us closer to the ultimate goal of the project: restoring the natural production of Pacific lampreys in the Umatilla River to self-sustaining and harvestable levels.  
The CTUIR project has been instrumental in developing a restoration plan for Pacific lamprey in the mid-Columbia drainage by collecting information on: past and current lamprey abundance; factors limiting production; habitat requirements for each life phase; donor stock availability; population genetics; disease; migratory pheromones; migratory timing; and homing information. In addition, research conducted by CTUIR staff has provided valuable information on the biology and ecology of Pacific lamprey within the basin.
The project began in 1995 to explore why the once-abundant Pacific lamprey (Lampetra tridentata) populations were declining in mid-upper Columbia and Snake River tributaries (Close et al. 1995). As a valuable food and cultural resource for Native Americans of the Pacific Northwest, the declining populations of lamprey impacted treaty-secured fishing opportunities by forcing the four Columbia River treaty tribes to gather this traditional food fish in lower Columbia River locations (Close et al. 1995).  
The Umatilla River basin was chosen by the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) as the initial pilot restoration project for several reasons: 1) the Umatilla River historically produced a fishable population of lampreys, 2) restoration efforts for salmonids in the basin may help with overall Pacific lamprey restoration, and 3) current population level of Pacific lampreys in the Umatilla River were extremely low. In 1998, CTUIR began developing a restoration plan. Historical abundance and distribution data were summarized from tribal and ODFW interviews, and larval abundance and distribution surveys were completed in the John Day, Umatilla, Walla Walla, Tuccannon, and Grand Ronde rivers. In cooperation with NPPC, Columbia River Intertribal Fish Commission (CRITFC), U.S. Geological Survey (Columbia River Research Laboratory, (CRRL)), USFWS, and ODFW, CTUIR staff developed the restoration plan for the basin. Knowledge on historical abundance, lamprey handling, transport, spawning, and outplanting of larvae were collected, and experiments conducted to maximize the potential for the restoration of lampreys in the Umatilla River.
In 2000, CTUIR implemented a pilot project in the Umatilla River. Outplantings of adult lampreys in the Umatilla River began in May 2000, and the monitoring of several metrics of lamprey (larval densities, number of upmigrating adults and outmigrating larvae) has continued annually. Now in its fourth year, the pilot project continues to outplant sexually mature adult lamprey in an effort to increase larval densities in the upper basin.
In 2005 we will continue efforts to broaden the research and restoration priorities of Pacific lamprey by continuing to outplant broodstock to the upper Umatilla basin and monitor various population metrics. We will also continue our efforts to identify stress related steroids in Pacific lamprey, and to understand the population genetics by microsatellite DNA techniques.
Additionally, we will introduce a new component to our research efforts by exploring the functional role of larval lamprey in the Umatilla, North Fork and John Day rivers. As larval lamprey abundance increases in the Umatilla River as a direct result of the four years of outplanting efforts, understanding their role in the ecosystem may provide insight into changes in water quality and benthic resource availability. As suspension feeders that remain in the substrate from 4-6 years, larval lamprey can remove large amounts of particulate matter from the water column, and transfer those resources to the substrate as biodeposits. Therefore, larval lamprey may have important effects on water quality and benthic resources.
The project has the following objectives for 2005:
1.  Increase larval abundance in the Umatilla River by outplanting adult                       lampreys.
2.  Estimate lamprey abundance before and after outplanting adults in the Umatilla River.
3.  Determine the presence/absence of Pacific lamprey migratory pheromone in the Umatilla River throughout the year.
4.  Quantitatively assess the functional role of Pacific lamprey in the Umatilla and John Day basins.
5.  Characterize stress related steroids in Pacific lamprey.
6.  Determine the population genetics of Pacific lamprey.
7.  Report results to the funding agency.
8.  Publish findings in peer-reviewed journals and attend professional conferences.
9.  Convert Statement of Work for Jan. 1, 2005 - Dec. 31, 2005 into the Work Element Format.
GOAL
The overall goal of this research is to provide essential information through implementation of a recovery plan for Pacific lamprey in the Umatilla River. In addition, research conducted by CTUIR staff has provided valuable information on the biology and ecology of Pacific lamprey within the basin These objectives will provide information that will be useful for restoration efforts elsewhere in the Columbia River Basin where lamprey may be declining or extirpated.  

References used in Work Elements:
Barker, J.H.A., Matthes, M., Arnold, G.M., Edwards, K.J., Ahman, I., Larsson, S., Karp, A., 1999.Characterization of genetic diversity in potential biomass willows (Salix spp.) by RAPD and AFLP analyses. Genome 42, 173-183.
Bryan MB, Libants SV, Warrillow JA, Li W and Scribner KT.  2002.  Polymorphic microsatellite markers for the landlocked sea lamprey, Petromyzon marinus.  In press Conservation Genetics.
Christian, A.D., D. J. Berg, and B. Crump. 2001.  Seasonal ecosystem processing and nutrient recycling of freshwater mussels in headwater streams.  Abstract from program, 2nd Symposium of the Freshwater Mollusc Conservation Society, March 12-14, 2001, Pittsburgh, PA. pp. 27-28.
Close D.A.  2001.  Effects of acute stress and tagging on the swimming performance and physiology of Pacific Lamprey (Lampetra tridentate).  Masters thesis, Oregon State
Conover, R.J. 1966. Assimilation of organic matter by zooplankton. Limnology and Oceanography 11:338-345.
Kreeger, D.A. and Langdon, 1993. C.J. Effect of dietary protein content on growth of juvenile mussels, Mytilus trossulus (Gould 1850). Biological Bulletin 185:123-139.
Liu, Z., Nichols, A., Li, P., Dunham, R.A., 1998. Inheritance and usefulness of AFLP markers in channel catfish (Ictalurus punctatus), blue catfish (I. furcatus), and their F1, F2 and backcross hybrids. Mol. Gen. Genet. 258, 260-268.
Mueller, U.G., Wolfenbarger, L.L., 1999. AFLP genotyping and fingerprinting. Trends in Ecology and Evolution 14, 389-394.
Raymond M, Rousset F (1995) GENEPOP (Version 1.2): Population genetics software for exact tests and ecumenicism.  Journal of Heredity 86, 248-249.
Seki, S., Agresti, J.J., Gall, G.A.E., Taniguchi, N., Bernie, M., 1999. AFLP analysis of genetic diversity in three populations of ayu Plecoglossus altivelis. Fisheries Science 65, 888-892.
Vos, P., Hogers, R., Bleeker, M., 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23, 4407-4414.
Wang, Z.Y., Wang, Y.L., Lin, L.M., Khoo, S.K., Okamoto, N., 2002. Genetic polymorphisms in wild and cultured large yellow croaker Pseudosciaena crocea using AFLP fingerprinting. J. Fish. Sci. China. 9, 198-202.
Zane, L., Bargelloni, L., Patarnello, T. 2002. Strategies for microsatellite isolation: a review. Molecular  Ecology 2002; 11(1): 1-16.