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This page provides details of the selected work element.

Work Element Summary PDF
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Contract Number:
29562
Contract Title:
200305400 GRN REPRO OF STEELHEAD IN HOOD RIVER
Contract Start Date:
10/1/2006
Contract End Date:
9/30/2007
WE ID:
157. Collect/Generate/Validate Field and Lab Data
Work Element Usage Report
Title:
C: 157 - Genotyping
Description:
Summarize statistics for fish genotyped to date, give progress assessment. Approximately 900 new fish genotyped by end of each reporting quarter.
WE Agreement Type:
Contracted
Deliverable Specification:
Genotype 3600 new fish. Includes the most recent year's samples plus fish from the backlog of samples from the 1990's. In particular, we will concentrate on finishing the last of the Hold summer run fish from the early 1990’s. A fish has been successfully genotyped following initial PCR of 8 microsatellite loci, running of products on an ABI 3100 capillary electrophoresis system, initial scoring of electrophoretic output, re-running and scoring for any failed reactions or ambiguous peaks, input of data into the master database, and error checking of the database. More details on loci and methods are explained in Araki et al., (2006; Conservation Biology, in press; http://oregonstate.edu/~blouinm/pdf_files/Araki%20et%20al%20In%20press%20Cons%20Biol.pdf ).
WSE Effective Budget:
$151,874
% of Total WSE Effective Budget:
54.83%
WSE Start:
10/01/2006
WSE End:
09/30/2007
WSE Completion:
WSE Progress:
Other
WSE ID Continued From:
n/a
WSE ID Continued To:
n/a
Finite or Recurring:
Recurring

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Budget History

SOWRevision Planned Updated Contractor Comments (optional) BPA Comments (optional)
Work Element Budget (Current Performance Period) $159,383 $151,874

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Milestones

6 Milestones
Sort Type Title Start End Status Modified By Modified Date
A EC Environmental compliance requirements complete 10/1/2006 10/2/2006 Concluded Tracy Hauser 8/8/2006 3:56:09 PM
Description: On-the-ground work associated with this work element cannot proceed until this milestone is complete. Milestone is complete when final documentation is received from BPA environmental compliance staff (completion can be based on pre-existing environmental documentation from BPA).
B approximately 900 fish genotyped 10/1/2006 12/31/2006 Concluded Michael Blouin 8/7/2006 5:13:10 PM
Description: document genotyping of fish in quarterly report
C approximately 900 fish genotyped 1/1/2007 3/31/2007 Concluded Michael Blouin 8/7/2006 5:13:10 PM
Description: document genotyping of fish in quarterly report
D approximately 900 fish genotyped 4/1/2007 6/30/2007 Concluded Michael Blouin 8/7/2006 5:13:11 PM
Description: document genotyping of fish in quarterly report
E approximately 900 fish genotyped 7/1/2007 9/30/2007 Active Michael Blouin 8/7/2006 5:13:11 PM
Description: document genotyping of fish in final report
F DELIV Successfully genotype approximately 3600 fish 9/30/2007 Active Michael Blouin 8/24/2006 5:50:58 PM
Description: Genotype 3600 new fish. Includes the most recent year's samples plus fish from the backlog of samples from the 1990's. In particular, we will concentrate on finishing the last of the Hold summer run fish from the early 1990’s. A fish has been successfully genotyped following initial PCR of 8 microsatellite loci, running of products on an ABI 3100 capillary electrophoresis system, initial scoring of electrophoretic output, re-running and scoring for any failed reactions or ambiguous peaks, input of data into the master database, and error checking of the database. More details on loci and methods are explained in Araki et al., (2006; Conservation Biology, in press; http://oregonstate.edu/~blouinm/pdf_files/Araki%20et%20al%20In%20press%20Cons%20Biol.pdf ).

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Metrics

Implementation Metrics
Metric Planned Actual Contractor Comments Measures HLIs
1464. Primary R, M, and E Focal Strategy [Population Status, Hydrosystem, Tributary Habitat, Estuary/Ocean, Harvest, Hatchery, Predation, Multiple Strategies] Multiple Strategies
1472. Primary R, M, and E Type [Status and Trend Monitoring, Action Effectiveness Research, Uncertainties Research, Project Implementation/ Compliance Monitoring] Uncertainty Research

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Location

Work Site ID Latitude Longitude State County Province Subbasin
7400 45.602053 -121.635814 Oregon Hood River Columbia Gorge Hood

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Focal Species

Primary Focal Species:
Steelhead (O. mykiss) - Lower Columbia River DPS (Threatened)
Secondary Focal Species:
None

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Environmental Compliance

None
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RM&E Metadata

Data Repositories:
""
Study Plan:
Study Plan Name Study Plan Owner Protocol State Sample Design Name
BPA Fish and Wildlife Program Monitoring v1.0 Russell Scranton Draft Genotyping - Oregon State University: Hood River v1.0

N/A

N/A

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