55849: Rev 1: WE G: Work Element Summary

Work Element Summary PDF

Select a work element:

Basics

Contract Number:

55849

Contract Title:

2003-063-00 EXP REPRO SUCCESS ABERNATHY CREEK

Contract Start Date:

1/1/2012

Contract End Date:

12/31/2012

WE ID:

162. Analyze/Interpret Data

Work Element Usage Report

Title:

G: 162 - Estimate genetic change for an integrated steelhead broodstock

Description:

Objective 2
This objective provides an opportunity to gauge the efforts initiated in the hatchery protocols to minimize the genetic hazards associated with a hatchery supplementation program, and to identify temporal changes or trends in genetic variability that may occur within and among the HOR and NOR components of the steelhead population in Abernathy Creek. Indices of genetic diversity will be compared between samples of steelhead collected from the raceways just prior to release and samples of outmigrating steelhead collected from the screw trap in Abernathy Creek.

WE Agreement Type:

Contracted

Deliverable Specification:

Annual comparisons of multi-locus DNA genotype information from 150 HOR and 150 NOR steelhead will be used to estimate genetic change between HOR and NOR steelhead from Abernathy Creek. Selectively-neutral DNA marker information will be used to determine the amount of genetic change occuring in a HOR/NOR integrated steelhead stock produced and reared in a conservation hatchery as compared to NOR steelhead.

WSE Effective Budget:

$20,712

% of Total WSE Effective Budget:

3.56%

WSE Start:

01/01/2012

WSE End:

11/30/2012

WSE Completion:

11/30/2012

WSE Progress:

Concluded

WSE ID Continued From:

n/a

WSE ID Continued To:

n/a

Finite or Recurring:

Recurring

Budget History

SOWRevision	Planned	Updated	Contractor Comments (optional)	BPA Comments (optional)
Work Element Budget (Current Performance Period)	$20,712	$20,712

Milestones

3 Milestones

Sort	Type	Title	Start	End	Status	Modified By	Modified Date
A	PUBPROTOCOL	Review, revise, and Publish protocol, study design, and methods in monitoringmethods.org	1/1/2012	1/31/2012	Concluded	Denise Hawkins	9/22/2011 9:36:32 AM
		Description: The Protocol (including temporal and spatial design) and Methods for this work element are stored at monitoringmethods.org and need to be finalized (i.e., "Published" through monitoringmethods.org), preferably prior to data collection. Preparations for contract renewals must include reviewing any previously published Protocols/Methods to ensure that they are consistent with how work will be done in any subsequent contract.
B		Compare genetic variability in HOR and NOR smolts	6/1/2012	11/30/2012	Concluded	Denise Hawkins	9/21/2011 1:21:38 PM
		Description: Multi-locus DNA genotypes - 150 BY11 HOR and 150 outmigrating NOR steelhead smolts - will be used to determine similarity between HOR and NOR.
C	DELIV	Estimate genetic change for an integrated steelhead broodstock		11/30/2012	Concluded	Denise Hawkins	9/21/2011 10:10:28 AM
		Description: Annual comparisons of multi-locus DNA genotype information from 150 HOR and 150 NOR steelhead will be used to estimate genetic change between HOR and NOR steelhead from Abernathy Creek. Selectively-neutral DNA marker information will be used to determine the amount of genetic change occuring in a HOR/NOR integrated steelhead stock produced and reared in a conservation hatchery as compared to NOR steelhead.

Metrics

Implementation Metrics

Metric	Planned	Actual
1464. Primary R, M, and E Focal Strategy [Population Status, Hydrosystem, Tributary Habitat, Estuary/Ocean, Harvest, Hatchery, Predation, Multiple Strategies]	Multiple Strategies	Hatchery
1472. Primary R, M, and E Type [Status and Trend Monitoring, Action Effectiveness Research, Uncertainties Research, Project Implementation/ Compliance Monitoring]	Uncertainty Research	Uncertainty Research
1495. Secondary R, M, and E Focal Strategy [Population Status, Hydrosystem, Tributary Habitat, Estuary/Ocean, Harvest, Hatchery, Predation, Multiple Strategies]	Hatchery	NULL

Location

<a target='blank' href='https://www.monitoringresources.org/Designer/Design/Detail/5547'>Location data for Area of Inference (AOI) should be entered into MonitoringResources.org. Click here to go to that site.</a>

Focal Species

Primary Focal Species:

Steelhead (O. mykiss) - Lower Columbia River DPS (Threatened)

Secondary Focal Species:

None

Environmental Compliance

This work element does not require Environmental Compliance

RM&E Metadata

Data Repositories:

Title	Link
NONE - No data repository or not electronically available	N/A

Study Plan:

Study Plan Name	Study Plan Owner	Protocol State	Sample Design Name
ExpireDetermine multi-locus DNA genotypes for hatchery- and natural-origin steelhead smolts and estimate genetic change for the integrated broodstock. v1.0	Patricia Crandell	Draft	Estimate genetic change for an integrated steelhead broodstock - US Fish and Wildlife Service (USFWS): Abernathy Creek Expired v1.0

Methods:

ID	Name	Type	Optional	Customized Based On	Status
380	Adult Run Timing v1.0	Data Analysis/Interpretation	No	N/A	Finalized
454	Adult Sex Ratio v1.0	Data Collection	No	N/A	Finalized
384	Calculating Relative Contribution v1.0	Data Analysis/Interpretation	No	N/A	Finalized
372	Estimation of Hatchery Strays v1.0	Data Analysis/Interpretation	No	N/A	Finalized
447	Fish Salvage v1.0	Data Collection	No	N/A	Finalized
385	Size at return v1.0	Data Analysis/Interpretation	No	N/A	Draft

Metrics:

Title	Category	Subcategory	Subcategory focus 1	Subcategory focus 2
	Fish	Abundance of Fish (ID: 46)	Fish Life Stage: Adult - Spawner	NA
	Fish	Abundance of Fish (ID: 46)	Fish Life Stage: Adult - Pre-Spawner	Fish Origin: Both
	Fish	ELISA Values: Fish (ID: 66)	NA	NA
Heterozygosity	Fish	Genetics: Fish Diversity, Fitness or Variation (ID: 72)	Fish Origin: Both	NA
	Fish	Hatchery Practices: Propagation (ID: 87)	Fish Origin: Both	NA
	Fish	Relative Reproductive Success (RRS) (ID: 88)	Fish Origin: Both	NA
	Fish	Survival Rate: Fish (ID: 99)	Fish Life Stage: RANGE: Egg to Juvenile	Fish Origin: Both
	Fish	Passage Efficiency (ID: 248)	Fish Life Stage: Adult - Pre-Spawner	NA

Area of Inference:

Name	Value
HUC4 - Sub Basin	LOWER COLUMBIA-CLATSKANIE
HUC6 - Sub Watershed	Abernathy Creek

Please correct the errors noted below.

Implementation Metrics