55849: Rev 1: WE T: Work Element Summary

Work Element Summary PDF

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Basics

Contract Number:

55849

Contract Title:

2003-063-00 EXP REPRO SUCCESS ABERNATHY CREEK

Contract Start Date:

1/1/2012

Contract End Date:

12/31/2012

WE ID:

162. Analyze/Interpret Data

Work Element Usage Report

Title:

T: 162 - Determine the relative reproductive success of NOR and HOR steelhead in Abernathy Creek above AFTC

Description:

Objective 7
For a supplementation program to be successful in contributing to the recovery of a small population in decline, the HOR component of a population released to spawn naturally must produce offspring. Comparing relative reproductive success (RRS) between HOR and NOR components of a population is one way to assess fitness differences. We will determine the parentage of NOR juvenile steelhead collected from above the AFTC weir and compare the number of juveniles with HOR parentage to the number of juveniles with NOR parentage.

WE Agreement Type:

Contracted

Deliverable Specification:

Using a set of highly variable, mircosatellite nuclear DNA loci and parentage analysis, we will determine the relative reproductive success of NOR and HOR steelhead in Abernathy Creek. Reproductive success will be estimated by determining the proportion of juveniles (parr and smolts), sampled from Abernathy Creek above the AFTC weir that were parented by each adult fish type (NOR and HOR).

WSE Effective Budget:

$31,407

% of Total WSE Effective Budget:

5.40%

WSE Start:

01/01/2012

WSE End:

11/30/2012

WSE Completion:

11/30/2012

WSE Progress:

Concluded

WSE ID Continued From:

n/a

WSE ID Continued To:

n/a

Finite or Recurring:

Recurring

Budget History

SOWRevision	Planned	Updated	Contractor Comments (optional)	BPA Comments (optional)
Work Element Budget (Current Performance Period)	$31,407	$31,407

Milestones

3 Milestones

Sort	Type	Title	Start	End	Status	Modified By	Modified Date
A	PUBPROTOCOL	Review, revise, and Publish protocol, study design, and methods in monitoringmethods.org	1/1/2012	1/31/2012	Concluded	Denise Hawkins	9/22/2011 9:43:06 AM
		Description: The Protocol (including temporal and spatial design) and Methods for this work element are stored at monitoringmethods.org and need to be finalized (i.e., "Published" through monitoringmethods.org), preferably prior to data collection. Preparations for contract renewals must include reviewing any previously published Protocols/Methods to ensure that they are consistent with how work will be done in any subsequent contract.
B		Conduct pedigree reconstruction analyses	8/1/2012	11/30/2012	Concluded	Denise Hawkins	9/21/2011 3:05:15 PM
		Description: Using a set of highly variable, mircosatellite nuclear DNA loci and parentage analysis, we will determine the relative reproductive success of NOR and HOR steelhead in Abernathy Creek. Reproductive success will be estimated by determining the proportion of juveniles (parr and smolts), sampled from Abernathy Creek above the AFTC weir that were parented by each adult fish type (NOR and HOR).
C	DELIV	Determine the relative reproductive success of NOR and HOR steelhead in Abernathy Creek above AFTC		11/30/2012	Concluded	Denise Hawkins	9/21/2011 3:05:15 PM
		Description: Using a set of highly variable, mircosatellite nuclear DNA loci and parentage analysis, we will determine the relative reproductive success of NOR and HOR steelhead in Abernathy Creek. Reproductive success will be estimated by determining the proportion of juveniles (parr and smolts), sampled from Abernathy Creek above the AFTC weir that were parented by each adult fish type (NOR and HOR).

Metrics

Implementation Metrics

Metric	Planned	Actual
1464. Primary R, M, and E Focal Strategy [Population Status, Hydrosystem, Tributary Habitat, Estuary/Ocean, Harvest, Hatchery, Predation, Multiple Strategies]	Multiple Strategies	Hatchery
1472. Primary R, M, and E Type [Status and Trend Monitoring, Action Effectiveness Research, Uncertainties Research, Project Implementation/ Compliance Monitoring]	Uncertainty Research	Uncertainty Research
1495. Secondary R, M, and E Focal Strategy [Population Status, Hydrosystem, Tributary Habitat, Estuary/Ocean, Harvest, Hatchery, Predation, Multiple Strategies]	Hatchery	Population Status

Location

<a target='blank' href='https://www.monitoringresources.org/Designer/Design/Detail/9616'>Location data for Area of Inference (AOI) should be entered into MonitoringResources.org. Click here to go to that site.</a>

Focal Species

Primary Focal Species:

Steelhead (O. mykiss) - Lower Columbia River DPS (Threatened)

Secondary Focal Species:

None

Environmental Compliance

This work element does not require Environmental Compliance

RM&E Metadata

Data Repositories:

Title	Link
NONE - No data repository or not electronically available	N/A

Study Plan:

Study Plan Name	Study Plan Owner	Protocol State	Sample Design Name
AFTC - Natural Reproductive Success and Demographic Effects of Hatchery-Origin Steelhead in Abernathy Creek, Washington v1.0	Patricia Crandell	Draft	Determine the relative reproductive success of NOR and HOR steelhead in Abernathy Creek above AFTC - US Fish and Wildlife Service (USFWS) v1.0

Methods:

ID	Name	Type	Optional	Customized Based On	Status
380	Adult Run Timing v1.0	Data Analysis/Interpretation	No	N/A	Finalized
454	Adult Sex Ratio v1.0	Data Collection	No	N/A	Finalized
485	Cormack-Jolly-Seber (CJS) Model v1.0	Data Analysis/Interpretation	No	N/A	Finalized
1353	DNeasy® 96 Fin tissue DNA Extraction Kit Protocol v1.0	Data Collection	No	N/A	Finalized
118	Electrofishing - Backpack - Mark v1.0	Data Collection	No	N/A	Finalized
119	Electrofishing - Backpack - Recapture v1.0	Data Collection	No	N/A	Finalized
115	Electrofishing - Determine Electrofisher Settings v1.0	Data Collection	No	N/A	Finalized
117	Electrofishing - Fish Processing and Recovery v1.0	Data Collection	No	N/A	Finalized
116	Electrofishing - Site Selection v1.0	Data Collection	No	N/A	Finalized
195	Estimating Adult Spawner Abundance v1.0	Data Collection	No	N/A	Finalized
1345	Estimating Genetic Diversity v1.0	Data Analysis/Interpretation	No	N/A	Finalized
696	Estimating relative reproductive success (RRS) v1.0	Data Analysis/Interpretation	No	N/A	Finalized
372	Estimation of Hatchery Strays v1.0	Data Analysis/Interpretation	No	N/A	Finalized
700	Fin tissue sampling for genetic analysis v1.0	Data Collection	No	N/A	Finalized
447	Fish Salvage v1.0	Data Collection	No	N/A	Finalized
653	Gill Na+/K+ATPase activity v1.0	Data Collection	No	N/A	Finalized
499	Hatchery Fish Marking and Tagging v1.0	Data Collection	No	N/A	Draft
453	Hatchery Propagation v1.0	Data Collection	No	N/A	Archived
550	Juvenile fish survival v1.0	Data Analysis/Interpretation	No	N/A	Draft
358	Mark Retention v1.0	Data Collection	No	N/A	Draft
4002	Microsatellite genotyping of Oncorhynchus mykiss using standardized SPAN loci v1.0	Data Collection	No	N/A	Finalized
446	Number of Hatchery Smolts v1.0	Data Collection	No	N/A	Finalized
133	Rotary Screw Trap Deployment and Operation v1.0	Data Collection	No	N/A	Finalized
388	Size-at-Emigration v1.0	Data Analysis/Interpretation	No	N/A	Draft
289	Statistical Testing for Changes in Adult Abundance v1.0	Data Analysis/Interpretation	No	N/A	Finalized
134	Trap Efficiency Testing v1.0	Data Collection	No	N/A	Finalized
145	Weirs v1.0	Data Collection	No	N/A	Finalized

Metrics:

Title	Category	Subcategory	Subcategory focus 1	Subcategory focus 2
Fish: Abundance of fish	Fish	Abundance of Fish (ID: 46)	Fish Life Stage: Adult Fish	Fish Origin: Both
Fish: Genetic Diversity of Populations	Fish	Genetics: Fish Diversity, Fitness or Variation (ID: 72)	Fish Origin: Both	NA
Fish: Genetics, diversity, fitness, variation	Fish	Genetics: Fish Diversity, Fitness or Variation (ID: 72)	Fish Origin: Both	NA
Fish: Hatchery practices, propagation	Fish	Hatchery Practices: Propagation (ID: 87)	Fish Origin: Both	NA
Fish: Relative reproductive success	Fish	Relative Reproductive Success (RRS) (ID: 88)	Fish Origin: Both	NA
Fish: Survival rate egg to juvenile	Fish	Survival Rate: Fish (ID: 99)	Fish Life Stage: RANGE: Egg to Juvenile	Fish Origin: Both
Fish: Passage efficiency, adult prespawner	Fish	Passage Efficiency (ID: 248)	Fish Life Stage: Adult - Pre-Spawner	NA
Fish: Spawning/nesting	Fish	Spawning/Nesting (ID: 507)	Fish Origin: Natural	NA

Area of Inference:

Name	Value
HUC6 - Sub Watershed	Abernathy Creek
HUC4 - Sub Basin	LOWER COLUMBIA-CLATSKANIE

Please correct the errors noted below.

Implementation Metrics