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A | 155663 | 165 | Produce Environmental Compliance Documentation | Produce Environmental compliance document for kelt activities | Secure environmental coverage documents from NOAA Fisheries for all steelhead activities. This work is done by CRITFC.
The NOAA permit that we received in early 2015 covers us through the end of the year. In January 2016 we will get a new permit that will cover January through June 2016 activities. | $5,000 | 0.31% | 07/01/2015 | 06/30/2016 |
B | 155664 | 66 | Trap/Collect/Hold/Transport Fish - Hatchery | Collect, hold, and transport kelt steelhead for experiments | (Objectives 2-5)
Post-spawn steelhead are found throughout the Columbia River Basin at various collection points. Steelhead in the kelt life stage will be collected for studies associated with objectives 2,3,4,and 5 of this project. Collections will be made at the following locations: Dworshak National Fish Hatchery for Objective 5 to evaluate progeny and gamete viability from reconditioned kelt steelhead; Yakima River for Objectives 2 and 3 to evaluate long term reconditioning and reproductive success in the wild; Snake and Clearwater rivers for Objectives 3 and 4 to evaluate long term reconditioning and study the physiology and endocrinology of steelhead kelts with a goal of evaluating the feasibility and success of several strategies for rehabilitating and handling of steelhead.
Work in this work element is done by CRITFC, YN, UofI, and Nez Perce Tribe. | $500,000 | 31.16% | 07/01/2015 | 06/30/2016 |
C | 155665 | 157 | Collect/Generate/Validate Field and Lab Data | Collect biological data and tissue for genotyping from adult steelhead | (Objectives 2-5)
Collect biological data including tissue samples from adult steelhead collected at weirs and traps. Data such as length, weight, condition, marks present, tag code will be stored in a database. Tissue samples will be stored in vials filled with ethanol or dried in coin envelopes. Samples will be labeled and cross referenced with tag codes. Collection locations are available under the location tab.
Work in this work element is done by CRITFC, YN, UofI, and Nez Perce Tribe. | $50,000 | 3.12% | 07/01/2015 | 06/30/2016 |
D | 155666 | 158 | Mark/Tag Animals | PIT tag adult steelhead at collection sites | (Objective 3)
All adult steelhead (including kelts) collected will be scanned for PIT tags and if no tag is found, a tag will be inserted. PIT tag identification codes will be cross-referenced with biological data and tissue samples.
Passive Integrated Transponder (PIT) tags will be implanted in the fish's pelvic girdle for identification. Only kelts determined to be in good or fair morphological condition will be used in the reconditioning program because research at Prosser Hatchery in 2000 indicated that those kelts collected in poor morphological condition did not survive captivity (Evans et al. 2001). This will allow us to individually identify fish allocated to different treatments.
Work in this work element is done by CRITFC, YN, UofI, and Nez Perce Tribe. | $25,000 | 1.56% | 07/01/2015 | 06/30/2016 |
E | 155667 | 176 | Produce Hatchery Fish | Recondition kelt steelhead for long term treatments | (Objectives 2-5)
Kelt steelhead will be reared for this project at a variety of locations and for a variety of reasons. Long-term reconditioning will occur in association with Objectives 2, 3, and 5 at Prosser Hatchery, Dworshak Hatchery. Rearing for experiments associated Objective 4 will occur at Dworshak National Hatchery.
Work in this work element is done by CRITFC, YN, UofI, and Nez Perce Tribe. | $35,000 | 2.18% | 07/01/2015 | 06/30/2016 |
F | 155668 | 157 | Collect/Generate/Validate Field and Lab Data | Collect survival, condition, and rematuration data on reared kelt steelhead | (Objectives 2-5)
Biological data will be collected from each specimen at the conclusion of rearing to aid in evaluation of the treatment. These data include blood collection, fork length, mid-eye to hypural length, weight, PIT tag number, condition, maturation status and gender.
Biological data will be observed and recorded for each specimen collected. Prior to release, surviving kelt will be anesthetized and examined with plasma hormone levels to determine maturation. Such data may be used for comparison between hatchery-origin and natural-origin individual since it has been reported that hatchery-origin fish tend to produce smaller eggs (Heath et al. 2003). Data such as PIT tag number, length, weight, marks, and condition by individual will be recorded.
Work in this work element is done by CRITFC, YN, UofI, and Nez Perce Tribe. | $37,500 | 2.34% | 07/01/2015 | 06/30/2016 |
G | 155669 | 162 | Analyze/Interpret Data | Analyze kelt steelhead post rearing data | (Objectives 2-5)
At the conclusion of the long-term reconditioning trials, data on fish condition, length, weight, and maturation status will be reported and compared to in-processing statistics. Work in this work element is done by CRITFC, YN, and Nez Perce Tribe.
Analysis of biological data to address the following hypotheses
Ho: Kelt steelhead reconditioning rates are similar spatially and temporally; and,
Ho: Kelt steelhead rematuration rates are similar spatially and temporally. | $20,000 | 1.25% | 07/01/2015 | 06/30/2016 |
H | 155670 | 157 | Collect/Generate/Validate Field and Lab Data | Genotype tissue samples from adult and kelt steelhead, and from resident O. mykiss | (Objective 2)
Resident trout can contribute to the anadromous cohort from a stream and therefore it is important to attempt to account for juveniles with undetermined linage by surveying resident populations. Collect resident O. mykiss for genotyping: collections of resident trout will be made using backpack electrofishing units, seining, trapping, or other suitable means. Collections of juvenile steelhead will also be made to relate back to parental type. Scale pattern analysis will be used to estimate age or length frequency analysis will be used to infer age of individuals sampled.
Work in this work element is done by CRITFC, YN. | $70,000 | 4.36% | 07/01/2015 | 06/30/2016 |
I | 155671 | 162 | Analyze/Interpret Data | Conduct parentage assignments, and stock identification from genetic data | (Objective 2)
Data will be analyzed with two specific goals: 1) to quantify gene flow between adult wild, hatchery and kelt steelhead within and between sites, using traditional population genetics tests (Hardy-Weinberg equilibrium, F statistics, assignment tests) [Genepop (Raymond and Rousset 1995); GDA (Lewis and Zaykin 1999)], and 2) to assign parentage of individuals based upon genotypes from 10-12 microsatellite loci. Maximum likelihood (Marshall et al. 1998) and Bayesian (Neff et al. 2001; Lange 1997) procedures will be used to exclude possible crosses and parents (parental exclusion analysis). The software program FaMoz (Gerber et al. 2003) will be used for this analysis.
Work in this work element is done by CRITFC. | $20,000 | 1.25% | 07/01/2015 | 06/30/2016 |
J | 155672 | 157 | Collect/Generate/Validate Field and Lab Data | Collect gamete and progeny viability data from kelt steelhead | (Objective 5)
Data on steelhead gamete and progeny viability. The treatment group will be consist of up to 50 steelhead at Dworshak Hatchery. Specifically, the following parameters and variables will be measured to assess and compare egg viability from the same experimental fish following maiden and repeat spawning (reconditioned):
1) Proportion of eggs within lots that reach 2-cell/4-cell stages (percent fertilization)
2) Proportion of eggs within lots that successfully complete early development
3) Proportion of eggs within lots that hatch
4) Egg diameter may also be measured and compared between virgin (first) and reconditioned (second) spawnings, to evaluate variability in egg size associated with reconditioning.
Work in this work element is done by CRITFC and the UofI.
Additional work will be done in the Cle Elum spawning channel where reconditioned kelts will be placed in the channel and allowed to spawn. Spawning success will be evaluated by assigning progeny to adults using genotypes. | $15,000 | 0.93% | 07/01/2015 | 06/30/2016 |
K | 155673 | 162 | Analyze/Interpret Data | Interpret gamete and progeny viability data from kelt steelhead | (Objective 5)
Interpret gamete and progeny viability data. Data will be collected in a repeated measures design, where data on gamete and progeny will be collected on DNFH steelhead from their first spawning, and following artificial reconditioning.
Work in this work element is done by CRITFC with collaboration with Nez Perce Tribe, and U of I. | $20,000 | 1.25% | 07/01/2015 | 06/30/2016 |
L | 155674 | 157 | Collect/Generate/Validate Field and Lab Data | Implement Snake River Kelt Reconditioning Plan | In this WE I've included descriptions of most of the work that is occurring in the Snake River Basin regarding kelt steelhead reconditioning. This is included to provide a narrative of activities however, much of this work is included in milestones from previous WEs). Presentations from this work will be made at regional and national conferences.
This project will study the physiology and endocrinology of steelhead kelts with a goal of evaluating the feasibility and success of several strategies for rehabilitating and handling of steelhead captured at Lower Granite Dam or at other sites during their downstream migration in the Snake River system. Our research will focus on the physiology, health and condition of both B and A stocks of steelhead. Through this research we will pose, develop and test protocols that can be used to collect and transport spent spawners, rehabilitate them for the most effective period of time to maximize their ability and contribution to the next spawning generation. This study is designed to develop the background science needed for evaluating different production plans for rehabilitation of kelts.
This work will include subcontracts to the University of Idaho and the Nez Perce Tribe.
SR1: Establish and validate assays for plasma and tissue level bioindicators of reproductive status, growth, and stress in steelhead kelts and post-spawning rainbow trout.
SR2: Use plasma vitellogenin and estradiol levels to assess the maturation status of female kelts in reconditioning programs prior to release. Provide maturation status to project managers.
SR3: Conduct a feeding experiment to evaluate the effectiveness of a supplemented diet in increasing survival, growth, and rematuration in long term reconditioning.
SR4: Determine the sequence of events in reproductive rematuration in post-spawning rainbow trout. Compare reproductive rematuration in post-spawning rainbow trout and kelt steelhead.
SR5: Determine whether administration of a glucocorticoid receptor antagonist reduces stress and increases growth in post-spawning rainbow trout.
This work is conducted by CRITFC, Nez Perce Tribe, and the University of Idaho. | $600,000 | 37.39% | 07/01/2015 | 06/30/2016 |
M | 155675 | 196 | Council Step Process | Snake River Kelt Master Plan | The Columbia Basin Accords includes funding for capital facilities for steelhead kelt reconditioning. This WE will support the development of a Master Plan for kelt reconditioning in the Snake River. This work will be completed by the Nez Perce Tribe under subcontract. CRITFC may hire an additional consultant to assist the Nez Perce Tribe with completing this task if necessary. Work began on this WE in 2009 and a draft has been developed. However, additionally drafting is likely and coordination meeting between co-managers need to be held to the complete the document prior to submitting it to the Council.
Approximately 70% complete. | $50,000 | 3.12% | | 06/30/2016 |
N | 155676 | 161 | Disseminate Raw/Summary Data and Results | Present findings with presentations | The strength of this project lies in the fact that it provides results from a series of tests to evaluate methods for enhancing iteroparity in wild steelhead populations in the Basin. Subsequent development of a suite of management recommendations, based on replicated, geographically representative tests of these methods, constitutes an equally important asset and product from this research. The purpose of this research is to definitively test research scenarios, resulting in a definitive set of empirically supported management options to enhance wild steelhead in the Basin.
Management recommendations will be developed following analysis, evaluation, and interpretation in a collaborative manner by a number of tribal, agency, and academic scientists, research, managers, culturists, pathologists, and geneticists. The collaborative nature of this proposed research is designed to produce a suite of definitive, scientifically defensible management recommendations to enhance populations of wild steelhead in the Columbia River Basin. | $15,000 | 0.93% | 07/01/2015 | 06/30/2016 |
O | 155677 | 183 | Produce Journal Article | Work toward submitting a peer-reviewed journal article | This project contains a lot of new and original work that is suitable for publication. In order to increase credibility and project value we will work toward publishing manuscripts in peer-reviewed journals.
Subjects of our envisioned manuscripts include: aquaculture considerations for reconditioning kelt steelhead; Yakima River steelhead run characteristics and kelt reconditioning efforts (in review); comparing gamete and progeny from maiden and repeat spawnings steelhead; bioenergetics of steelhead migrations; and, describing the series of physiological changes that occur during long term reconditioning in DNFH captive steelhead kelts. | $35,000 | 2.18% | 07/01/2015 | 06/30/2016 |
P | 155678 | 174 | Produce Plan | Hatchery Reconditiong Plan | We will draft a plan that describes the number of steelhead kelts collected and reconditioned at each facility, along with mortality, and release numbers. | $5,000 | 0.31% | 07/01/2015 | 06/30/2016 |
Q | 155679 | 119 | Manage and Administer Projects | Manage project and subcontracts for kelt project | Statements of work, budgets, invoices, billings, payments, accruals and other contract related documents will be produced. Contract negotiations will be undertaken. | $72,000 | 4.49% | 07/01/2015 | 06/30/2016 |
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